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401432 Goat Anti-Human IgG, H & L Chain F(ab’)₂ Fragment Peroxidase Conjugate

Goat Anti-Human IgG, H & L Chain F(ab’)₂ Fragment Peroxidase Conjugate: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
401432
  
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      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Adsorbed against bovine, horse, and mouse serum proteins.
      Catalogue Number401432
      Brand Family Calbiochem®
      References
      Product Information
      FormLyophilized
      FormulationLyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1:5000-1:100,000)
      Frozen Sections (1:500-1:5000)
      Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
      Immunocytochemistry (1:500-1:5000)
      Paraffin Sections (1:500-1:5000)
      Application CommentsBased on immunoelectrophoresis, this antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. Does not react with non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse, and mouse serum proteins. Antibody may, however, react with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenHuman IgG
      ImmunogenHuman
      HostGoat
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 500 µl dH₂O. Centrifuge if not completely clear after solubilizing for 2 h at room temperature. Following reconstitution, refrigerate (4°C). Undiluted stock solution is stable for up 6 weeks at 4°C. For long-term storage, add an equal volume of sterile glycerol and freeze (-20°C) (Note: addition of glycerol will decrease the protein and buffer concentration by half). Following the addition of glycerol, stock solution is stable for up to 1 year at -20°C. Diluted antibody is stable for up to 1 day at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Número de referencia GTIN
      401432 0

      Documentation

      Goat Anti-Human IgG, H & L Chain F(ab’)₂ Fragment Peroxidase Conjugate Certificados de análisis

      CargoNúmero de lote
      401432
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationELISA (1:5000-1:100,000)
      Frozen Sections (1:500-1:5000)
      Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
      Immunocytochemistry (1:500-1:5000)
      Paraffin Sections (1:500-1:5000)
      DescriptionImmunoaffinity purified goat polyclonal supplied as the F(ab')2 fragment conjugated to horseradish peroxidase. Recognizes human IgG, heavy and light chains.
      HostGoat
      Immunogen speciesHuman
      ImmunogenHuman IgG
      IsotypeIgG
      Specieshuman
      FormLyophilized
      FormulationLyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.
      PreservativeNone
      CommentsBased on immunoelectrophoresis, this antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. Does not react with non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse, and mouse serum proteins. Antibody may, however, react with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 500 µl dH₂O. Centrifuge if not completely clear after solubilizing for 2 h at room temperature. Following reconstitution, refrigerate (4°C). Undiluted stock solution is stable for up 6 weeks at 4°C. For long-term storage, add an equal volume of sterile glycerol and freeze (-20°C) (Note: addition of glycerol will decrease the protein and buffer concentration by half). Following the addition of glycerol, stock solution is stable for up to 1 year at -20°C. Diluted antibody is stable for up to 1 day at 4°C.
      Toxicity Standard Handling