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325888 NEK2, GST-Fusion, Human, Recombinant, S. frugiperda

NEK2, GST-Fusion, Human, Recombinant, S. frugiperda: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: (Never in mitosis gene A)-related protein kinase 2, HSPK 21, NimA-related protein kinase 2

325888
  
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Descripción

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human NEK2 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. NEK2 is a member of a serine/threonine-protein kinase family that plays an important role in mitotic regulation.
Catalogue Number325888
Brand Family Calbiochem®
Synonyms(Never in mitosis gene A)-related protein kinase 2, HSPK 21, NimA-related protein kinase 2
References
ReferencesSchultz, S.J., et al. 2004. Cell Growth Differ. 5, 625.
Hames, R.S. and Fry, A.M. 2002. Biochem. J. 361, 77.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to myelin basic protein per min at 30°C using variable concentrations from of ATP (0.1-7.6 µM).
EC number2.7.1.37
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
Applications
Biological Information
Specific Activity≥15 U/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Número de referencia GTIN
325888 0

Documentation

NEK2, GST-Fusion, Human, Recombinant, S. frugiperda Certificados de análisis

CargoNúmero de lote
325888

Referencias bibliográficas

Visión general referencias
Schultz, S.J., et al. 2004. Cell Growth Differ. 5, 625.
Hames, R.S. and Fry, A.M. 2002. Biochem. J. 361, 77.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2007 JSW
Synonyms(Never in mitosis gene A)-related protein kinase 2, HSPK 21, NimA-related protein kinase 2
DescriptionRecombinant, human NEK2 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. NEK2 is a member of a serine/threonine-protein kinase family that plays an important role in mitotic regulation.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
96-Well Membrane Binding Kinase Activity Assay Protocol:
Materials Required • Standard Kinase Assay Buffer: 125 mM HEPES-NaOH, 7.5 mM MgCl2, 7.5 mM MnCl2, 7.5 µM sodium -orthovanadate, 2.5 mM DTT, pH 7.5 • 10X Kinase Dilution Buffer: 500 mM HEPES-NaOH, 2.5 mg/ml PEG20.000, 10 mM DTT, pH 7.5 • Substrate: myelin basic protein, 5 µg/50 µl final concentration • NEK2, GST-Fusion, Human, Recombinant, S. frugiperda, diluted in 1X Kinase Dilution Buffer to desired concentration (we use 200 ng in a 50 µl reaction) • 33P-γ-ATP • ATP Mix: 1 µM ATP in H2O with 1 x 106 cpm/5 µl • 96-well V-bottom MTP (polypropylene) plates (Nunc Cat. No. 442587) • 96-well Multiscreen Vacuum Manifold (Millipore Cat. No. MAVM096OR) • 96-well Multiscreen Filterplates, phosphocellulose membrane (Millipore Cat. No. MSPH N6B50) and glass fiber (Millipore Cat. No. MSFC N6B50) • 2% H3PO4 • 0.9% NaCl
Activity Assay Protocol 1. In a polypropylene V-bottom plate add 20 µl Kinase Assay Buffer 2. If desired, add 5 µl test substance (e.g., inhibitor, activator, etc.; in 10% DMSO) 3. Add 10 µl Substrate (diluted in H2O). 4. Add 10 µl diluted NEK2. 5. Add 5 µl ATP Mix. 6. Mix on a shaker and incubate at 30°C for 80 min. 7. Stop the reaction with 50 µl H3PO4. 8. Prewet the filter plate with 200 µl H2O. 9. Filter the reaction mix by applying vacuum. 10. Wash 3 times with 200 µl 0.9% NaCl. 11. Add 200 µl scintillation fluid to each well and count on a scintillation counter.
EC number2.7.1.37
Specific activity≥15 U/mg protein
Unit definitionOne unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to myelin basic protein per min at 30°C using variable concentrations from of ATP (0.1-7.6 µM).
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesSchultz, S.J., et al. 2004. Cell Growth Differ. 5, 625.
Hames, R.S. and Fry, A.M. 2002. Biochem. J. 361, 77.