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524576 Phosphatase, Alkaline, Calf Intestine, Molecular Biology Grade

Dephosphorylation of nucleic acids

Phosphatase, Alkaline, Calf Intestine, Molecular Biology Grade: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: CIAP

524576
  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Overview

This product has been discontinued.

Please refer to our complete listing of alkaline phosphatases for possible alternatives. Please read the alternative product documentation carefully and contact technical service if you need additional information.





Native alkaline phosphatase from calf intestine. Calf Intestine Alkaline Phosphatase catalyzes the hydrolysis of 5'-terminal phosphates of DNA, RNA, and deoxy- and ribonucleoside triphosphates. This preparation is suitable for dephosphorylation of vector DNA to prevent “empty vector” religation in cloning experiments, and for removal of 5'-phosphate groups in preparation for labeling with polynucleotide kinase.
Catalogue Number524576
Brand Family Calbiochem®
SynonymsCIAP
References
ReferencesMoessner, E., et al. 1980. J. Physiol. Chem. 361, 543.
Product Information
CAS number9001-78-9
Activity≥1000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of <i>p</i>NPP per min at 37°C, pH 9.8.
EC number3.1.3.1
FormLiquid
FormulationIn 25 mM Tris-HCl, 1 mM MgCl₂, 100 µM ZnCl₂, 50% glycerol, pH 7.6.
Applications
Biological Information
Specific Activity≥2000 units/mg
Physicochemical Information
ContaminantsDNases, RNases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Número de referencia GTIN
524576 0

Documentation

Phosphatase, Alkaline, Calf Intestine, Molecular Biology Grade Certificados de análisis

CargoNúmero de lote
524576

Referencias bibliográficas

Visión general referencias
Moessner, E., et al. 1980. J. Physiol. Chem. 361, 543.

Folleto

Cargo
Protein Phosphatases Technical Bulletin
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2008 RFH
SynonymsCIAP
DescriptionNative alkaline phosphatase from calf intestine. Calf Intestine Alkaline Phosphatase catalyzes the hydrolysis of 5'-terminal phosphates of DNA, RNA, and deoxy- and ribonucleoside triphosphates. This preparation is suitable for dephosphorylation of vector DNA to prevent “empty vector” religation in cloning experiments, and for removal of 5'-phosphate groups in preparation for labeling with polynucleotide kinase. Suitable for use in reactions containing nucleotide sugars. Has an optimal pH of 8.0-10.5.
FormLiquid
FormulationIn 25 mM Tris-HCl, 1 mM MgCl₂, 100 µM ZnCl₂, 50% glycerol, pH 7.6.
CAS number9001-78-9
EC number3.1.3.1
ContaminantsDNases, RNases: none detected
Specific activity≥2000 units/mg
Activity≥1000 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of pNPP per min at 37°C, pH 9.8.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesMoessner, E., et al. 1980. J. Physiol. Chem. 361, 543.