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70049 pBACgus-2cp DNA

70049
  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Overview

This product has been discontinued.





pBACgus-2cp is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. The plasmid also carries the gus gene encoding b-glucuronidase under control of the late basic protein promoter (P6.9), which serves as a reporter to verify recombinant viruses by staining with X-gluc. pBACgus-2cp provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. A Ligation-Independent Cloning (LIC) version of the vector is available for rapid, directional cloning of PCR products adjacent to the enterokinase cleavage site. The vector provides optional expression of a C-terminal His•Tag fusion sequence by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map.
Catalogue Number70049
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Número de referencia GTIN
70049 0

Documentation

pBACgus-2cp DNA Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

pBACgus-2cp DNA Certificados de análisis

CargoNúmero de lote
70049

Folleto

Cargo
Insect Cell Expression

Citas

Título
  • Carla V. Finkielstein, Michael Overduin and Daniel G. Capelluto. (2006) Cell migration and signaling specificity is determined by Rac1's phosphatidylserine recognition motif. Journal of Biological Chemistry 281, 27317-27326.
  • Mapa vectorial

    Cargo
    TB141VM pBACgus-2cp Vector Map

    Secuencia vectorial

    Cargo
    pBACgus-2cp Sequence