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69658 pLysE DNA

pLysE DNA: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
69658
  
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Descripción

Replacement Information
Description
Overview

This product has been discontinued.





pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Catalogue Number69658
Brand Family Novagen®
References
References1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
Product Information
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Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
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Specifications
Global Trade ITEM Number
Número de referencia GTIN
69658 0

Documentation

pLysE DNA Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

pLysE DNA Certificados de análisis

CargoNúmero de lote
69658

Referencias bibliográficas

Visión general referencias
1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.

Citas

Título
  • Mark S. McClain and Timothy L. Cover. (2007) Functional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin. Infection and Immunity 75, 1785-1793.
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    Mapa vectorial

    Cargo
    TB107 pLysS and pLysE Plasmid Map

    Secuencia vectorial

    Cargo
    pLysE Sequence