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MABT1529-100UG Anti-Cytokeratin 8 Antibody, clone 35betaH11

Anti-Cytokeratin 8, clone 35betaH11, Cat. No. MABT1529, is a mouse monoclonal antibody that detects Cytokeratin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABT1529-100UG
100 μg  
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Replacement Information
Description
Catalogue NumberMABT1529-100UG
DescriptionAnti-Cytokeratin 8 Antibody, clone 35betaH11
Alternate Names
  • Keratin
  • type II cytoskeletal 8
  • CK-8
  • Keratin-8
  • K8
  • Type-II keratin Kb8
Background InformationKeratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central alpha-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. It is a type II, neutral to basic protein that together with cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is shown to be highly conserved in all type II keratins. Phosphorylation at Serine 73 is reported to increase during cellular stress. However, under normal conditions serine 73 remains largely dephosphorylated. It can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Cytokeratin 8, clone 35betaH11, Cat. No. MABT1529, is a mouse monoclonal antibody that detects Cytokeratin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Cytokeratin-8 in human prostate tissue sections.

Western Blotting Analysis: A representative lot detected Cytokeratin-8 in Western Blotting applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Cytokeratin-8 in Immunohistochemistry applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).
Biological Information
ImmunogenA cytoskeletal preparation of the human hepatocellular carcinoma cell line Hep3B.
Clone35betaH11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 35betaH11 is a mouse monoclonal antibody that specifically detetcts human Cytokeratin 8.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KRT8
  • CYK8
Purification MethodProtein G purified
UniProt Number
Molecular Weight53.70 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Cytokeratin-8 in human pancreas tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Référence GTIN
MABT1529-100UG 04054839997358

Documentation

Anti-Cytokeratin 8 Antibody, clone 35betaH11 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Cytokeratin 8 Antibody, clone 35betaH11 Certificats d'analyse

TitreNuméro de lot
Anti-Cytokeratin 8, clone 35betaH11 - Q3236794 Q3236794

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues.
Gown, AM; Vogel, AM
Am J Pathol  114  309-21  1983

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