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CA1028 Anti-c-Myb/v-Myb Mouse mAb (4E3)

Anti-c-Myb/v-Myb Mouse mAb (4E3) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
CA1028
  
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      Aperçu

      Replacement Information

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      Description
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      This product has been discontinued.





      Recognizes the ~ 80 kDa c-Myb/v-Myb protein in Jurkat and LS174T cells.
      Catalogue NumberCA1028
      Brand Family Calbiochem®
      Application Data
      Detection of human c-Myb/v-Myb by immunohistochemistry. Sample: Human seminoma. Primary antibody: Anti-c-Myb/v-Myb Mouse mAb (4E3) (Cat. No. CA1028) (2 µg/ml). Detection: fluorescence.

      Detection of human c-Myb/v-Myb by immunoblotting. Sample: Human c-Myb/v-Myb. Primary antibody: Anti-c-Myb/v-Myb Mouse mAb (4E3) (Cat. No. CA1028) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesSleaman, J.P., et al. 1993. Oncogene 8, 1931.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlJurkat or Ls174T cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (2-4 µg/ml)
      Paraffin Sections (2-4 µg/ml; heat pre-treatment required, see comments)
      Application CommentsStaining of formalin-fixed, paraffin-embedded tissues requires boiling the tissue section in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant v-Myb
      ImmunogenHuman
      Clone4E3
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Référence GTIN
      CA1028 0

      Documentation

      Références bibliographiques

      Aperçu de la référence bibliographique
      Sleaman, J.P., et al. 1993. Oncogene 8, 1931.

      Brochure

      Titre
      Antibody Sourcebookﾙ Sixth Edition
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationImmunoblotting (2-4 µg/ml)
      Paraffin Sections (2-4 µg/ml; heat pre-treatment required, see comments)
      Application Data
      Detection of human c-Myb/v-Myb by immunohistochemistry. Sample: Human seminoma. Primary antibody: Anti-c-Myb/v-Myb Mouse mAb (4E3) (Cat. No. CA1028) (2 µg/ml). Detection: fluorescence.

      Detection of human c-Myb/v-Myb by immunoblotting. Sample: Human c-Myb/v-Myb. Primary antibody: Anti-c-Myb/v-Myb Mouse mAb (4E3) (Cat. No. CA1028) (2 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~80 kDa c-Myb/v-Myb protein.
      BackgroundMyb is a transcription factor expressed in hematopoietic cells and immature gastroepithelial cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant v-Myb
      Clone4E3
      IsotypeIgG2a
      Specieshuman
      Positive controlJurkat or Ls174T cells
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsStaining of formalin-fixed, paraffin-embedded tissues requires boiling the tissue section in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°) for long-term storage.
      Toxicity Standard Handling
      ReferencesSleaman, J.P., et al. 1993. Oncogene 8, 1931.