Get on the fast track
to production
Introducing the VirusExpress® AAV Production Platform
Gene therapy developers have compressed timelines that require the manufacture of AAV vector as quickly as possible. We’re here to help.
Our new VirusExpress® 293 AAV Production Platform offers a transfection-based solution using a HEK293 suspension adapted cell line, chemically defined medium, and a clinically relevant process dramatically reducing time to commercial production. Our VirusExpress® System offers you a higher vector yield than adherent platforms and a simpler process than competitors. The VirusExpress® System has been optimized for AAV2, AAV5, and AAV6 production.
With a platform that can reduce process development and scale-up time, whether performed by leveraging our gene therapy CDMO capabilities or in your own facility, you can draw on our experience to speed your gene therapy to patients.
To learn about our VirusExpress® Production Platform for Lentivirus or access ordering information, visit our full portfolio offering.
VirusExpress® AAV Production Platform Performance
Bioreactor Growth
VirusExpress® 293 AAV Production Cells were cultured for batch growth in Mobius® 50 L Single-Use Bioreactors. Cells were seeded at 6 x 105 cells/mL and diluted with fresh media on Day 3. Peak viable cell density (VCD) of 8 x 106 cells/mL was obtained in Mobius® 50L bioreactor with viability >90%.
Productivity by Serotype
VirusExpress® 293 AAV Production Cells – productivity shown as crude harvest genome titers (gc/mL) of GFP model virus in shake flasks using multiple serotypes, AAV2, AAV5 and AAV6.
Productivity Using Alternative Transfection Reagents
VirusExpress® 293 AAV Production Cells – AAV2 crude harvest genome titers (gc/mL) of GFP model virus in shake flasks using PEI based transfection versus alternative transfection reagents.
Productivity Improvements
VirusExpress® 293 AAV Production Cells – AAV2 production with a cationic polymer transfection reagent consistently delivers a 2-fold increase in genome titers (gc/mL) of GFP model virus when compared to PEI mediated transfection at the 3 L bioreactor scale (solid color bars) versus shake flask controls (striped color bars).
EVALUATE TODAY
Contact us today to learn how our VirusExpress® AAV Production Platform can help you get on the fast track to production.
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