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Merck

B6404

BCIP®/NBT solution, premixed

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About This Item

NACRES:
NA.32
UNSPSC Code:
41105333

Key Documents

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form

liquid

Quality Level

100

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Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

BCIP/NBT solution, premixed has been used:
  • as a substrate to develop dot-blots
  • as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
  • to detect specific immune complexes in western blot analysis

General description

Substrate for visualizing alkaline phosphatase conjugates in Western blotting.

Other Notes

0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Alcohols + Hazardous rank II

fsl

B6404PROC: + B6404-100ML: + B6404-BULK: + B6404-100ML-KC: + B6404-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000284841
0000284841
SLCJ4826
SLCD5988
SLBX5444

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Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
Tsai R K, et al.
Experimental Eye Research, 87(3), 242-250 (2008)
Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Slow-and fast-twitch rat hind limb skeletal muscle phenotypes 8 months after spinal cord transection and olfactory ensheathing glia transplantation
Negredo P, et al.
The Journal of Physiology, 586(10), 2593-2610 (2008)
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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