QR0200
Quantitative RT-PCR ReadyMix™
One step RT-qPCR for probe-based methods, MMLV & hot-start Taq
Synonym(s):
1-step RT-qPCR mix, Quantitative real-time PCR master mix
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About This Item
usage
sufficient for 250 reactions
feature
dNTPs included, hotstart
technique(s)
RT-qPCR: suitable
color
colorless
input
purified RNA
compatibility
ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000
detection method
probe-based
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
Application
Features and Benefits
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced risk of contamination from multiple pipetting steps
- Reduced set-up time as compared to manual or wax Hot Start methods
- JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
- Broad instrument compatibility
- Includes a separate ROX reference dye vial for reaction normalization
General description
Legal Information
Packaging
Kit Components Only
- Probe Based qRT-PCR ReadyMix 2 X
- Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U
signalword
Warning
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
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prtr
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fsl
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ishl_indicated
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ishl_notified
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Articles
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
遺伝子発現分析における課題には、mRNA安定性、時間的転写パターン、mRNAとタンパク質の相関などがあり、正確さに影響を及ぼしています。
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
Protocols
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Related Content
GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.
RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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