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Merck

07-436

Anti-monomethyl-Histone H3 (Lys4) Antibody

Upstate®, from rabbit

Synonym(s):

H3K4me1, Histone H3 (mono methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, vertebrates
Application:
ChIP, DB, ICC, IF, WB, inhibition assay
Technique(s):
ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable
Citations:
177
Uniprot accession no.:
Q16695

Key Documents

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biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

monomethylation (Lys4)

Quality Level

100

Gene Information

human ... H3C1(8350)

Analysis Note

Control
HeLa cell extract
Routinely evaluated by Western Blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.

Application

Anti-monomethyl-Histone H3 (Lys4) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H3 (Lys4) also known as H3K4me1, Histone H3 (mono methyl K4) & has been validated in ICC, IF, PIA, WB, ChIP-seq, DB.
Immunocytochemistry:
Reported by an independent laboratory.

Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Biochem/physiol Actions

Broad species to cross-reactivity is expected.
Recognizes monomethyl-histone H3 (Lys4), Mr 17kDa.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa

Immunogen

Epitope: Lys4
KLH-conjugated, synthetic peptide containing the sequence ART[meK]…, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.

Preparation Note

Stable for 1 year at 2 to 8°C from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

07-436:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Th2 LCR is essential for regulation of Th2 cytokine genes and for pathogenesis of allergic asthma.
Koh, BH; Hwang, SS; Kim, JY; Lee, W; Kang, MJ; Lee, CG; Park, JW; Flavell, RA; Lee, GR
Proceedings of the National Academy of Sciences of the USA null
Min Gyu Lee et al.
Methods (San Diego, Calif.), 40(4), 327-330 (2006-11-15)
Histone methylation is involved in the regulation of many cellular processes. In the past 2 years, several histone demethylases including BHC110/LSD1 have been characterized. BHC110, the first known histone lysine demethylase, removes methyl groups from methylated histone H3 lysine 4
PU.1 and C/EBP(alpha) synergistically program distinct response to NF-kappaB activation through establishing monocyte specific enhancers.
Jin, F; Li, Y; Ren, B; Natarajan, R
Proceedings of the National Academy of Sciences of the USA null
Transcriptional activation by pRB and its coordination with SWI/SNF recruitment.
Flowers, S; Beck, GR; Moran, E
Cancer Research null
Virion-mediated transfer of SV40 epigenetic information.
Milavetz, B; Kallestad, L; Gefroh, A; Adams, N; Woods, E; Balakrishnan, L
Epigenetics null
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Global Trade Item Number

SKUGTIN
07-43604053252364976

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