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Merck

AB5539

Anti-Neurofilament H (200 kDa) Antibody

CHEMICON®, chicken polyclonal

Synonym(s):

NF-H

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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Product Name

Anti-Neurofilament H Antibody, Chemicon®, from chicken

biological source

chicken

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, pig, bovine, feline, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

NM_021076.2

UniProt accession no.

P12036

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

Analysis Note

Control
Positive control: brain.

Negative control: kidney, liver.

Application

Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.

General description

200 kDa

Immunogen

Purified bovine NF-H.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified chicken IgY in PBS containing 5mM sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zu-Lin Chen et al.
Developmental biology, 327(1), 158-168 (2009-01-03)
Laminins have dramatic and varied actions on neurons in vitro. However, their in vivo function in brain development is not clear. Here we show that knockout of laminin gamma1 in the cerebral cortex leads to defects in neuritogenesis and neuronal
The neurotoxicological effects of mastoparan Polybia-MPII at the murine neuromuscular junction: an ultrastructural and immunocytochemical study.
Thalita Rocha, Bibiana M de Souza, Mario S Palma, Maria Alice da Cruz-Hofling, John Buchanan Harris
Histochemistry and Cell Biology null
Neuromuscular toxicology of the venom of Collett's snake (Pseudechis colletti): a histopathological study.
Andrew J Hart,Wayne C Hodgson,Tracey Scott-Davey,John B Harris
Muscle and Nerve null
Combined application of brain-derived neurotrophic factor and neurotrophin-3 and its impact on spiral ganglion neuron firing properties and hyperpolarization-activated currents.
Karina Needham,Bryony A Nayagam,Ricki L Minter,Stephen J O'Leary
Hearing Research null
Cardioprotection induced by Na+/K+-ATPase activation involves extracellular signal-regulated kinase 1/2 and phosphoinositide 3-kinase/Akt pathway.
Zheng J, Koh X, Hua F, Li G, Larrick JW, Bian JS
Cardiovascular Research null
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Global Trade Item Number

SKUGTIN
AB553904053252506338

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