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Merck

MAB4703

Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18

clone 4-1-18, Chemicon®, from mouse

Synonym(s):

CPP32

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4-1-18, monoclonal
Application:
IHC, WB
Citations:
8

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4-1-18, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_004346.3, NM_032991.2

UniProt accession no.

P42574

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)

Immunogen

Epitope: large subunit & proform
Recombinant full-length human caspase-3 protein

Application

Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18 detects level of Caspase 3 & has been published & validated for use in WB, IH(P).
Research Category
Apoptosis & Cancer

Metabolism
Research Sub Category
Caspases

Enzymes & Biochemistry
Western blot: 1:1000. The antibody will detect a band at 32 kDa corresponding to the inactive unprocessed form of caspase 3 as well as a 17-22 kDa band corresponding to the large subunit of active caspase 3.

Immunohistochemistry (frozen and paraffin): 1:500.



Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes the full length 32 kDa caspase-3 protein. Highly specific to caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two smaller subunits of 17 kDa and 12 kDa. Caspase-3 is one of the most extensively studied enzyme in apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers.

Physical form

Format: Purified
Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Analysis Note

Control
POSITIVE CONTROL: 293 (human embryonic kidney) cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-439

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB4703:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elmira R Mordakhanova et al.
International journal of molecular sciences, 21(7) (2020-04-11)
Heparin-induced thrombocytopenia (HIT) is an adverse drug reaction characterized by thrombocytopenia and a high risk for venous or arterial thrombosis. HIT is caused by antibodies that recognize complexes of platelet factor 4 and heparin. The pathogenic mechanisms of this condition
Growth of primary embryo cells in a microculture system.
Villa M, Pope S, Conover J, Fan TH
Biomedical Microdevices null
Cassia alata leaf extract induces cytotoxicity in A549 lung cancer cells via a mechanism that is caspase 8 dependent.
A Levy,A Lewis
The West Indian Medical Journal null
The marine lipopeptide somocystinamide A triggers apoptosis via caspase 8.
Wrasidlo, W; Mielgo, A; Torres, VA; Barbero, S; Stoletov, K; Suyama, TL; Klemke et al.
Proceedings of the National Academy of Sciences of the USA null
Kui Wang et al.
Autophagy, 7(9), 966-978 (2011-05-26)
Quercetin, a dietary antioxidant present in fruits and vegetables, is a promising cancer chemopreventive agent that inhibits tumor promotion by inducing cell cycle arrest and promoting apoptotic cell death. In this study, we examined the biological activities of quercetin against

Global Trade Item Number

SKUGTIN
MAB470304053252359224

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