Skip to Content

Key Documents

View All Documentation

E4884

Ethylenediaminetetraacetic acid disodium salt dihydrate

ACS reagent, 99.0-101.0%

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
6381-92-6
Molecular Weight:
372.24
NACRES:
NA.77
PubChem Substance ID:
24894586
UNSPSC Code:
12352107
EC Number:
205-358-3
Beilstein/REAXYS Number:
3900609
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Segment

grade

ACS reagent

agency

suitable for SM 4500 - NH3

assay

99.0-101.0%

form

solid

reaction suitability

reagent type: chelator

impurities

≤0.005% Insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

pH

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

solubility

H2O: 100 g/L at 20 °C

cation traces

Fe: ≤0.01%, heavy metals: ≤0.005% (by ICP-OES)

application(s)

microbiology

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

General description

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:
  • of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
  • of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
  • of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Features and Benefits

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Still not finding the right product?

Explore all of our products under Ethylenediaminetetraacetic acid disodium salt dihydrate

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Codes

H332,H373

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

E4884-6X500G: + E4884-EW: + E4884-100G: + E4884-BULK: + E4884-CH: + E4884-6X100G: + E4884-VAR: + E4884-500G:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library