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Merck

X100PC

Triton™ X-100

peroxide- and carbonyl-free

Synonym(s):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
UNSPSC Code:
12161900
NACRES:
NA.25
PubChem Substance ID:
329830773
MDL number:
MFCD00128254
Beilstein/REAXYS Number:
2315025

Key Documents

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Product Name

Triton™ X-100, peroxide- and carbonyl-free

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

packaging

ampule of 5, 5x5, 10x5 mL

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

HLB

13.5

Quality Level

200

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Application

0·3% Triton™ X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Packaging

Packaged in ampules under Argon.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK5736
SLCH5409
SLCC6581
SLBL0870V
SLBJ8444V

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Adeno-associated virus mediated SOD gene therapy protects the retinal ganglion cells from chronic intraocular pressure elevation induced injury via attenuating oxidative stress and improving mitochondrial dysfunction in a rat model.
American Journal of Translational Research, 8(2), 799-799 (2016)
Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b.
Experimental Eye Research, 79(1), 131-140 (2004)
Cytotoxicity of bilirubin for human fibroblasts and rat astrocytes in culture. Effect of the ratio of bilirubin to serum albumin.
Chuniaud L, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 256(2), 103-114 (1996)
The size, shape, and hydration of nonionic surfactant micelles. Triton X-100.
Robson RJ and Dennis EA
The Journal of Physical Chemistry, 81, 1075-1078 (1977)
Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM).
Koley D and Bard AJ
Proceedings of the National Academy of Sciences of the USA, 107, 16783-16783 (2010)
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