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47670

Formamide

puriss. p.a., ACS reagent, ≥99.5% (GC/T)

Synonym(s):

Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
75-12-7
Molecular Weight:
45.04
EC Number:
200-842-0
UNSPSC Code:
12352111
PubChem Substance ID:
329757333
Beilstein/REAXYS Number:
505995
MDL number:
MFCD00007941
Assay:
≥99.5% (GC/T)
Technique(s):
PCR: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
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Quality Level

200

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

grade

ACS reagent, puriss. p.a.

assay

≥99.5% (GC/T)

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 %

technique(s)

PCR: suitable

impurities

≤0.02% free acid (as HCOOH), ≤0.1% water

refractive index

n20/D 1.447

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

transition temp

transition temp 2.0-3.0 °C

density

1.132 g/mL at 20 °C, 1.134 g/mL at 25 °C (lit.)

cation traces

Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Na: ≤20 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Zn: ≤1 mg/kg

suitability

in accordance for miscibility

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

General description

Formamide is a colorless organic solvent. It has been reported to increase the precision of PCR (Polymerase Chain Reaction) studies. Microwave spectral study of its isotopic forms suggests that the molecule of formamide is nonplanar in which the H2N-C group exhibits a shallow pyramid like structure.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

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pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

305.6 °F

flash_point_c

152 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class II Designated Chemical Substances

prtr

Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water soluble liquid

fsl

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

47670-250ML-F:4.548173373867E12 + 47670-2.5L-F:4.54817337385E12 + 47670-1L-F:4.548173373843E12

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J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is
Microwave spectrum and molecular structure of formamide.
Costain CC and Dowling JM.
J. Chem. Phys. , 32(1), 158-165 (1960)
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 32(1), 130-130 (1960)
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