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Merck

CMC0007

SIG10 5a Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(s):

Chemically Competent Cells

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About This Item

UNSPSC Code:
41106202
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped

Key Documents

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Product Name

SIG10 5a Chemically Competent Cells, for DNA plasmid production

biological source

Escherichia coli

grade

Molecular Biology

form

buffered aqueous solution

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 108 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols.
They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide solutions for a wide range of applications at economical prices.

Genotype

fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Features and Benefits

  • Direct replacements for standard cloning strains like DH5α™, DH10B, JM109, TOP10, XL1-Blue, etc.
  • Optimized genetics for high yields: endonuclease and recombination minus
  • Blue - white screening

Other Notes

  • SIG10 5a chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


Legal Information

DH5α is a trademark of Invitrogen Corp.

Storage Class

10 - Combustible liquids

wgk

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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