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D3035

Deoxyribonucleic acid from human placenta

Name: Deoxyribonucleic acid from human placenta
Brand: SIGMA

buffered aqueous solution, sexed, female

Synonym(s):

DNA

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About This Item

CAS Number:

9007-49-2

UNSPSC Code:

41106310

NACRES:

NA.52

MDL number:

MFCD00130921

eCl@ss:

32160414

Biological source:

human placenta

Form:

buffered aqueous solution

Storage temp.:

−20°C

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Properties

biological source

human placenta

Quality Segment

200

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

Description

General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:

to compare the efficiency of DNA quantification methods
as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
as an internal control to estimate the degree of fragmentation of circulating DNA
for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
to compare the 70-bp P5 exon sequence between DNA of different human and primate species
to calculate copy number of genes, relative to normal human tissue
in PCR reactions

For use in Southern hybridizations.

Other Notes

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D3035-.1MG: + D3035-VAR: + D3035-.5MG: + D3035-BULK:

jan

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