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F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):

flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
—
Citations:
6078
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Quality Segment

200

biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1.0-1.2 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F1804-50UG: + F1804-200UG: + F1804-BULK-N: + F1804-5MG: + F1804-1MG: + F1804-BULK: + F1804-SAMPLE: + F1804-VAR:

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