assay
≥94% (titration)
storage temp.
2-8°C
SMILES string
BrCCCBr.N(CCCCCCN(C)C)(C)C
InChI
1S/C10H24N2.C3H6Br2/c1-11(2)9-7-5-6-8-10-12(3)4;4-2-1-3-5/h5-10H2,1-4H3;1-3H2
InChI key
KZKAYEGOIJEWQB-UHFFFAOYSA-N
General description
Application
Hexadimethrine bromide has been used for lentivirus infection in cells. It has been used for infection of cells with retroviral supernatant.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Articles
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
