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Merck

H9268

Hexadimethrine bromide

≥94% (titration)

Synonym(s):

1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, Polybrene

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About This Item

CAS Number:
UNSPSC Code:
41106502
NACRES:
NA.26
MDL number:
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assay

≥94% (titration)

storage temp.

2-8°C

SMILES string

BrCCCBr.N(CCCCCCN(C)C)(C)C

InChI

1S/C10H24N2.C3H6Br2/c1-11(2)9-7-5-6-8-10-12(3)4;4-2-1-3-5/h5-10H2,1-4H3;1-3H2

InChI key

KZKAYEGOIJEWQB-UHFFFAOYSA-N

General description

Hexadimethrine bromide is a quaternary ammonium compound which works as a heparin antagonist. It is also involved in the transformation of low molecular weight DNA.

Application

Hexadimethrine bromide can be used to transfect mammalian cells with DNA. It can be used to increase the efficiency of lipofection transfections.
Hexadimethrine bromide has been used for lentivirus infection in cells. It has been used for infection of cells with retroviral supernatant.


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

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Articles

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

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