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L3540

Lowry Reagent

Name: Lowry Reagent
Brand: SIGMA

Synonym(s):

Copper(II) tartrate Mix

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

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Properties

Quality Level

100

Description

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.

For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.

Preparation Note

Reconstitute to 40 mL with deionized water.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

pictograms

GHS02,GHS05,GHS07

signalword

Danger

hcodes

H228,H302,H314,H412

pcodes

P210 - P260 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

L3540-1VL:4.548173403311E12

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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.