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M5755

MOPS-EDTA-Sodium Acetate (MESA)

Name: MOPS-EDTA-Sodium Acetate (MESA)
Brand: SIGMA

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

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Properties

grade

Molecular Biology

Quality Segment

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H₂O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

Description

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:

40 mM MOPS
10 mM sodium acetate
1 mM EDTA (pH 8.3)

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

M5755-1L:4.548173399546E12

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