N6019
Neutralizing Buffer
for Northern and Southern blotting, powder
Synonym(s):
Neutralizing Buffer for Blotting Techniques
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
grade
Molecular Biology
Quality Segment
form
powder
concentration
2.15-2.35 M chloride (by silver nitrate titration )
technique(s)
Southern blotting: suitable
foreign activity
DNase, RNase, none detected
storage temp.
room temp
General description
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
Application
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
Preparation Note
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
N6019-BULK: + N6019-VAR: + N6019-1L: + N6019-5X1L:
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