NA2110

GenElute^™ Bacterial Genomic DNA Kits

Name: GenElute<SUP>™</SUP> Bacterial Genomic DNA Kits
Brand: SIGMA

sufficient for 70 purifications

Synonym(s):

Bacterial Genomic DNA, bacterial DNA prep, bacterial DNA purification kit, bacterial gDNA extraction kit, bacterial gDNA isolation kit, bacterial gDNA purification kit, bacterial genomic DNA extraction kit, bacterial genomic DNA isolation kit, bacterial genomic DNA purification kit, Gen Elute

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About This Item

NACRES:

NA.55

UNSPSC Code:

41105501

Key Documents

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Product Name

GenElute^™ Bacterial Genomic DNA Kits, sufficient for 70 purifications

usage

sufficient for 70 purifications

technique(s)

DNA purification: suitable

Quality Level

200

test parameters

: 70—120 min kit run time
sample volume: 1.5 mL bacterial culture

storage temp.

15-25°C

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Related Categories

gDNA Purification

Nucleic Acid Purification

Application

The purified bacterial genomic DNA is ready for downstream applications such as:

restriction endonuclease digestions
PCR
Southern blots
cloning

Biochem/physiol Actions

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

Starting material: Up to 1.5 mL of culture
Expected yield: Up to 20 μg
Elution volume: 400 μl
Time required: 70 - 120 min
A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
No phenol, chloroform, or ethanol precipitation required

Typical DNA yields of 15 μg - 20 μg
Protocols provided for Gram + and Gram - bacteria
High quality genomic DNA in less than 2 hours
Purified DNA has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Click here to view the kit protocol

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS02,GHS08,GHS05,GHS07,GHS09

signalword

Danger

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

77.0 °F - closed cup

flash_point_c

25 °C - closed cup

wgk

WGK 3

hcodes

H226,H290,H302,H315,H318,H334,H335,H336,H410,EUH430

pcodes

P201 - P202 - P210 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

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Draft Whole-Genome Sequence of Bacillus sonorensis Strain L12, a Source of Nonribosomal Lipopeptides.

David B Adimpong et al.

Genome announcements, 1(2), e0009713-e0009713 (2013-03-30)

The Bacillus sonorensis L12 draft genome sequence is approximately 4,647,754 bp in size with a G+C content of 45.2%. Over 86% of the genome contains protein-encoding genes, including several gene clusters for de novo biosynthesis of the nonribosomal lipopeptides iturin, bacitracin

Quantitative PCR-based competitive index for high-throughput screening of Salmonella virulence factors.

Hyunjin Yoon et al.

Infection and immunity, 79(1), 360-368 (2010-11-03)

Salmonella enterica serovar Typhimurium is an intracellular pathogen and a main cause of food-borne illness. In this study, a quantitative PCR (qPCR)-based competitive index (CI) method was developed to simultaneously compare the growth of multiple Salmonella strains. This method was

An endophytic microbe from an unusual volcanic swamp corn seeks and inhabits root hair cells to extract rock phosphate.

Hanan R Shehata et al.

Scientific reports, 7(1), 13479-13479 (2017-10-19)

In the animal microbiome, localization of microbes to specific cell types is well established, but there are few such examples within the plant microbiome which includes endophytes. Endophytes are non-pathogenic microbes that inhabit plants. Root hairs are single cells, equivalent

Population and demographic structure of Ixodes scapularis Say in the eastern United States.

Joyce M Sakamoto et al.

PloS one, 9(7), e101389-e101389 (2014-07-16)

The most significant vector of tick-borne pathogens in the United States is Ixodes scapularis Say (the blacklegged tick). Previous studies have identified significant genetic, behavioral and morphological differences between northern vs. southern populations of this tick. Because tick-borne pathogens are

Antimicrobial mechanisms of ortho-phthalaldehyde action.

Manuel Simões et al.

Journal of basic microbiology, 47(3), 230-242 (2007-05-24)

Biocides generally have multiple biochemical targets. Such a feature easily entangles the analysis of the mechanisms of antimicrobial action. In this study, the action of the dialdehyde biocide ortho-phtalaldehyde (OPA), on bacteria, was investigated using the Gram-negative Pseudomonas fluorescens. The

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Articles

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Protocols

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute^™ Bacterial Genomic DNA Kits