P1598
PCR 20 bp Low Ladder
for electrophoresis of PCR fragments
Synonym(s):
20 bp Low Ladder, Marker for DNA gel electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
41141827
Biological source:
fermentation/recombinant (E. coli strain DH5A)
Form:
liquid
Solubility:
water: soluble at 20 °C
Storage temp.:
2-8°C
biological source
fermentation/recombinant (E. coli strain DH5A)
Quality Segment
grade
Molecular Biology
form
liquid
usage
80 uses
solubility
water: soluble at 20 °C
suitability
suitable for electrophoresis (DNA)
storage temp.
2-8°C
General description
Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Application
PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.
Other Notes
Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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