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Merck

P7582

Amino-terminal FLAG-BAP™ Fusion Protein

Synonym(s):

Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00130921

Key Documents

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form

liquid

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.
Learn more product details in our FLAG® application portal.

General description

Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Other Notes

Control protein

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK2898
SLCH4561
SLCG2086
SLBW2067
SLBZ4309

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Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
BAP-fusion: A versatile molecular probe for biotechnology research
Biotechnology: Research, Technology and Applications, 327-345 (2008)
Daniela Castiglia et al.
Biotechnology for biofuels, 9, 154-154 (2016-07-28)
Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of
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Articles

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Atto色素標識抗体を使用した蛍光マルチプレックス検出法

イムノブロッティング（ウェスタンブロット転写法）は現代のプロテオミクス研究において一般的な手法です。

Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

モノクローナル抗体アフィニティーゲルを使用したFLAG®融合タンパク質の免疫沈降

M2モノクローナル抗体4%アガロースアフィニティーゲルを使用したFLAG®融合タンパク質の免疫沈降（IP）のためのプロトコル

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タンパク質精製

イオン交換、サイズ排除、タンパク質アフィニティークロマトグラフィーなどの方法を用いた組換えタンパク質精製のためのタンパク質精製技術、試薬、プロトコル。

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Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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