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S2015

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonym(s):

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105319
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grade

Molecular Biology

Quality Segment

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Disclaimer

White precipitate/cloudiness may occur after cold shipping/storage due to salt crystallization (normal); re-dissolve by warming at 37 °C for 1–2 h with occasional swirling and use once clear.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

S2015-PH: + S2015PROC: + S2015-VAR: + S2015-1L-PW: + S2015-EW: + S2015-BULK: + S2015-1L:

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