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T9549

Thrombin from bovine plasma

powder, suitable for cell culture, ≥1,500 NIH units/mg protein (E1%/280 = 19.5)

Synonym(s):

Factor IIa

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About This Item

CAS Number:
9002-04-4
UNSPSC Code:
12352204
EC Number:
232-648-7
MDL number:
MFCD00166053
EC Number:
3.4.21.5 (BRENDA, IUBMB)
Specific activity:
≥1,500 NIH units/mg protein (E1%/280 = 19.5)
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form

powder

specific activity

≥1,500 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~31 kDa, light chain ~6 kDa

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P00735

shipped in

dry ice

storage temp.

−20°C

Gene Information

cow ... F2(280685)

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Preparation Note

When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units, and is measured by direct comparison to an NIH thrombin reference standard.
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T9549-50UN: + T9549-VAR: + T9549-PH: + T9549PROC: + T9549-100UN: + T9549-250UN: + T9549-500UN: + T9549-BULK:

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