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X4126

Xylene Cyanol FF

BioReagent, Molecular Biology

Synonym(s):

Acid blue 147, Cyanol FF, XC

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About This Item

Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
2650-17-1
Molecular Weight:
538.61
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
57654732
EC Number:
220-167-5
Colour Index Number:
42135
MDL number:
MFCD00019481
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grade

Molecular Biology

Quality Segment

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

H2O: complete 1 mg/mL, clear, blue

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

InChI key

VVLFAAMTGMGYBS-KRQUPCAFSA-M

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:
  • histological stain and as tracker dye in DNA sequencing and electrophoresis
  • gel-loading dye
  • formamide RNA loading dye
  • tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

X4126-VAR: + X4126-EW: + X4126-10G: + X4126-BULK:

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