YEAST1
Yeast Transformation Kit
reagents for introducing plasmid DNA into yeast
Synonym(s):
lithium acetate yeast transformation
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About This Item
grade
Molecular Biology
Quality Level
usage
kit sufficient for >100 standard transformations
technique(s)
transformation: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Features and Benefits
- Easy and ready-to-use
- Requires as little as 10 ng of plasmid DNA
- Flexibility for any strain of yeast
- Sufficient for over 100 standard transformations
Other Notes
- Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
- Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
- Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
- Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
- Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Please refer to KIT Component information
pdsc
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prtr
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fsl
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ishl_indicated
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ishl_notified
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Protocols
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
酵母は成長が速く、細胞が分散しているため、真核生物の研究に適したモデル生物と考えられています。
Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.
遺伝子工学により、研究のための遺伝子組換えタンパク質の大規模な発現・単離が可能になります。