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Merck

H6909

Hydrocortisone solution

50 μM, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

17-Hydroxycorticosterone, Cortisol

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About This Item

CAS Number:
50-23-7
NACRES:
NA.77
PubChem Substance ID:
24895778
UNSPSC Code:
12352209
MDL number:
MFCD00011654
Biological source:
non-animal source
Form:
liquid

Key Documents

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Product Name

Hydrocortisone solution, 50 μM, sterile-filtered, BioXtra, suitable for cell culture

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

SMILES string

C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

biological source

non-animal source

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

50 μM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

5.0- 7.0

shipped in

dry ice

storage temp.

−20°C

Quality Level

500

Gene Information

human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)

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Application

Hydrocortisone has been used in epithelial and endothelial adherent cell culture applications. Hydrocortisone solution has been used for the isolation of epidermal stem cells and cell expansion. It has also been used for keratinocyte permeability assay.

Other Notes

Animal-component free

Physical form

frozen in working aliquots, avoid repeated freeze/thaw

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK5842
SLCJ0893
SLCD6234
SLCB3001
SLBX8593

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Natalja E Fedorovich et al.
Biomacromolecules, 10(7), 1689-1696 (2009-05-19)
Application of hydrogels in tissue engineering and innovative strategies such as organ printing, which is based on layered 3D deposition of cell-laden hydrogels, requires design of novel hydrogel matrices. Hydrogel demands for 3D printing include: 1) preservation of the printed
A combination of aloe vera, curcumin, vitamin C, and taurine increases canine fibroblast migration and decreases tritiated water diffusion across canine keratinocytes in vitro.
Timothy R Fray et al.
The Journal of nutrition, 134(8 Suppl), 2117S-2119S (2004-07-31)
Antonio Celentano et al.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 50(3), 323-332 (2020-01-12)
Matrix metalloproteinases (MMPs) play a crucial role in the malignant phenotype of cancer cells. In particular, active levels of MMP2 in cancer cells have been associated with invasion and metastasis through the degradation of basement membrane extracellular matrix proteins. However
Xiao-hua Lei et al.
PloS one, 6(11), e26603-e26603 (2011-11-19)
The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as
Anita Golec et al.
STAR protocols, 3(2), 101419-101419 (2022-06-07)
Here, we present a standardized protocol for isolation, maintenance, and polarization of the respiratory epithelial primary cells from patient samples acquired from nasal brushing, polyp specimens, or lung explants. This protocol generates a clearly defined polarized layer of epithelial cells
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