Skip to Content
Merck
HomeProductsProtein BiologyProtein Electrophoresis & Western BlottingImmobilon Transfer Membranes & Kits

Immobilon Transfer Membranes & Kits

Immobilon® PVDF transfer membranes for Western blotting

Immobilon® PVDF transfer membranes provide high sensitivity, low background, broad solvent compatibility, and superior staining capabilities in Western blotting and other immunoblotting applications. Tailored for specific applications, detection chemistries, and blotting techniques, Immobilon® membranes are offered in a variety of options for optimal results, flexibility, and convenience.

Related Product Literature

Immobilon®-P PVDF Membranes

The original Immobilon® transfer membrane for Western blotting offers high protein adsorption and retention for greater sensitivity. To visualize the protein transfer efficiency, Immobilon®-P membrane may be stained with any reversible blot stain compatible with immunodetection (e.g., Ponceau-S,) or viewed by transillumination using a light box.

  • High protein binding for greater sensitivity
  • Durability for stripping and re-probing
  • 0.45 µm pore size ideal for most Western blotting applications
  • Compatible with absorbance (chromogenic) and chemiluminescent detection methods

Immobilon®-E PVDF Membranes

The Immobilon®-E membrane is the first PVDF membrane that wets out in aqueous buffers, eliminating the hazardous methanol pre-wet step.

  • Eliminates the hazardous methanol pre-wetting step associated with PVDF membranes
  • High protein binding capacity for better sensitivity
  • 0.45 µm pore size ideal for most Western blotting applications
  • Compatible with absorbance (colorimetric) and chemiluminescent detection methods

Immobilon®-FL PVDF Membranes

Immobilon®-FL membranes were optimized for fluorescence detection methods, featuring low background across a wide range of excitation and emission wavelengths.

  • Ideal for multiplexing, fluorescence, and chemifluorescence detection methods
  • Compatible with all commonly used fluorescent dyes
  • Also compatible with non-fluorescent detection chemistries
  • 0.45 µm pore size ideal for most Western blotting applications

Immobilon®-PSQ PVDF Membranes

Immobilon®-PSQ membranes were developed for protein sequencing and immunoblotting of low molecular weight proteins, offering highest binding capacity and retention for stronger signal. The 0.20 µm membrane pore size is ideal for retention of more proteins, reducing the risk of “blow-through” for protein targets with a molecular weight <20 kDa.

  • 0.2 µm pore size
  • Higher protein adsorption and sequencing yields compared to other membranes
  • Prevents blow-through of low molecular weight proteins
  • Recommended when blotting target proteins smaller than 20 kDa
  • Compatible with a variety of detection chemistries, including radioactive, chromogenic, and chemiluminescent techniques
Related Product Categories
Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Membrane Immunodetection Devices
Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.
Cytiva
Cytiva™ products for drug discovery research and therapeutics bioprocess manufacturing including Whatman™ lab filtration products, Amersham™ chromatography columns and resins, tissue culture reagents, and products for protein sample preparation, blotting, and analysis.
Nucleic Acid Electrophoresis, Transfer, and Quantitation
DNA and RNA gel electrophoresis reagents and Northern and Southern blotting tools for molecular biology and genomics research.
Related Applications
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Protein Expression
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein Pull-Down Techniques
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?