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Column	TSKgel UltraSW Aggregate 30 cm x 7.8 mm I.D., 3 µm particles (822856)
Column Temp.	Ambient
Mobile Phase	0.1 mol/L sodium phosphate buffer, pH 6.7, with an addition of either 0.2 mol/L arginine (A), or 0.2 mol/L proline (B)
Flow Rate	1.0 mL/min
Sample	mAB, aggregated at 75 °C for 5 min
Injection	20 µL
Detector	UV, 280 nm

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Analysis Note	While particle size, packing quality and column length are all important considerations in optimizing mAb aggregate analysis, mobile phase additives can also contribute to improving your separation. The addition of amino acids such as lysine and arginine to the mobile phase, increase aggregate recovery, while glutamine, proline and arginine improve monomer aggregate resolution, as well as monomer fragment resolution. Although the increases in resolution are not drastic, they confirm that increased resolution due to the use of an advanced mobile phase is possible and that mobile phase testing can contribute to a more reliable and robust aggregate analysis.
Categories	Analytical Chromatography, Antibodies
Featured Industry	Life Science and Biopharma
Legal Information	TSKgel is a registered trademark of Tosoh Corporation
Suitability	Application for HPLC

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