High-Resolution U/HPLC Separation of Monoclonal Antibodies with the BIOshell™ IgG 1000 Å Diphenyl

High resolution U/HPLC separation of monoclonal antibodies (mAbs), mAb variants, and other large proteins with orthogonal selectivity to alkyl-bonded phases.

The BIOshell™ IgG 1000 Å Diphenyl column, based on Fused-Core^® particles, is non-polar in nature. Due to the aromatic nature of the phase, both methanol and acetonitrile organic solvents should be evaluated in the separation; as acetonitrile can act as a much stronger solvent when aromatic ligands are employed in the stationary phase in reversed-phase separations. Typically, with mAb analysis workflows, column temperatures exceeding 60 °C are commonly employed; the BIOshell™ IgG 1000 Å Diphenyl column is compatible with such temperatures.

The BIOshell™ IgG 1000 Å Diphenyl column has shown unique advantages in resolving variants of antibody-drug conjugates (ADCs). This observation is most likely due to the interaction between the cytotoxic payload of the ADC with the diphenyl ligand.

BIOshell™ IgG 1000 Å Diphenyl columns are perfectly suitable for the separation of monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights ≤ 500 kDa with alternative selectivity to C4 and C18 column chemistries to ensure maximum resolution.

BIOshell™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core^® particle design. The Fused-Core^® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths.

Outstanding performance in U/HPLC. 2.7 µm SPP particle with 1000 Å pores. Ideal for the separation of large proteins.

BIOshell™ IgG 1000 Å Diphenyl Guard Cartridges

Finger-tight guard cartridge holder, Inlet
Provides easy replacement of guard cartrige.

Finger-tight guard cartridge holder, Outlet
Allows for replacement of cartridge without removing the guard holder from the flow path.

Titanium hybrid ferrule
Adds durability for multiple connections.

Auto-adjusting zero dead volume (ZDV) end fitting
Ensures optimum, ZDV connection to the analytical column.