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参考資料
| 参考資料の概要 | アプリケーション |
|---|---|
| MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues Omer Barad, Eti Meiri, Amir Avniel, Ranit Aharonov, Adi Barzilai, Isaac Bentwich, Uri Einav, Shlomit Gilad, Patrick Hurban, Yael Karov, Edward K. Lobenhofer, Eilon Sharon, Yoel M. Shiboleth, Marat Shtutman, Zvi Bentwich and Paz Einat Genome Research 14:2486-2494, 2004 2004 | |
| Highly selective membranes in protein ultrafiltration Feins M, Sirkar KK,Biotechnol Bioeng. 2004 Jun 20;86(6):603-11 Biotechnol Bioeng. 2004 Jun 20;86(6):603-11 2004 | Protein Purification |
| Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention Huisman IH (REPRINT) ; Pradanos P; Hernandez A Journal of membrane science 2000, V178, N1-2, p55-64 2000 2000 | |
| Integrated process for the removal of emulsified oils from effluents in the steel industry Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT) Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395 1999 | Industrial QC |
| Effect of Solution pH on Protein Transport Through Ultrafiltration Membranes Douglas B. Burns and Andrew L. Zydney Biotechnol Bioeng 64: 27-37, 1999. 1999 | |
| Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74 Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998 Journal of membrane science, v147, N2, p279-290 1998 1998 | Enzyme Assays |
| In vitro and in vivo grafting of xeno pig fetal liver fragments using ultrafiltration membrane Kanai N.; Morita N.; Munkhbat B.; Gansuvd B.; Hagihara M.; Nagamachi Y.;Tsuji K. Cell Transplantation SO- 7/4 (417-420) 1998 1998 | Cell Culture |
| Metabolic screening using on-line ultrafiltration mass spectrometry Van Breemen R.B.; Nikolic D.; Bolton J.L. Drug Metabolism and Disposition , 26/2 (85-90) 1998 1998 | |
| Performance Characteristics of Waste Oil Emulsion Treatment by Ultrafiltration Lin, S. H.; Lan, W. J. Yuan Ze University, Taoyuan, Taiwan J Environ Sci Health-Toxic Hazard Subst Environ Eng 1998,SO- vA33, n3, p385(20) 1998 | |
| General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification Lin, S.-C. Chen, Y.-C. Lin, Y.-M. J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998 1998 |
FAQ（よくある質問と回答）
| 質問 | 回答 |
|---|---|
| Are there any extractables in the UF disc membranes? | The YM, PM and XM membranes are treated with glycerin as a humectant. The glycerin bath contains sodium azide as a preservative. The membranes contain no other extractables. |
| As a rule of thumb, when separating two globular solutes, what size difference is required when performing ultrafiltration? | In general, a 10 fold difference is recommended. |
| Describe what happens when albumin (67,000 MW) and IgG (167,000 MW) are filtered through a 100,000 MW cut-off membrane? | The larger solute will form a gel layer preventing the smaller solute from completely passing. If processed separately, the IgG would be retained and the albumin would pass through the membrane. |
| How can I clean and restore the Ultracel and Biomax membranes after use? | Rinse the membrane for 30 minutes with either 0.1 M NaOH, 100 ppm NaOCl (at 25 degrees C), or 0.1 - 0.5% chemical cleaner. After rinsing, flush the membrane thoroughly with distilled or deionized water. |
| How effective is ultrafiltration for fractionation of protein solutions? | Ultrafiltration has limited utility for protein fractionation. Pore size distribution and membrane polarization effects contribute to the lack of separation precision. This is the reason for the wide spacing given to molecular weight cutoff points. Generally, an order of magnitude (10x) difference in molecule size is required to achieve reasonable separation. Separations may be enhanced by reducing the protein concentration, increasing agitation, or crossflow rate, or by optimizing buffers to minimize protein aggregation and adsorption. |
| Is the Ultracel YM membrane autoclavable? | Yes, the Ultracel YM membrane can be autoclaved, submerged in water, at 121 degrees C. |
| Which UF membrane do I want, Biomax or Ultracel? | Biomax is recommended for applications where very harsh pH conditions are required for processing or cleaning. Ultracel is recommended where harsh pH conditions are not needed and especially when protein loading is low (<20-g/m sq.) or the feedstock is highly fouling. Biomax is currently the premier UF membrane being used in Life Science applications, primarily due to its combination of low protein binding (i.e., high yield) and speed. Ultracel, Millipore’s new composite regenerated cellulose offering, is the lowest protein binding UF membrane available, making it ideal for dilute protein solutions where binding may be a significant factor in yield. For important applications, such as pilot trials prior to scale-up, it is recommended to conduct a trial with several pore sizes from each membrane family in order to determine which best meets the application criteria. |
| When doing diafiltration, how many initial volumes would it take to remove the salt? | It takes three initial volumes to remove 95% of the salt and five initial volumes to remove 99.9% of the salt. |
| What molecular weight cut-off should I select for proteins of different sizes? | As a rule, when using Ultracel membranes it is best to select a membrane 2-3 times smaller than the solute to be retained by the membrane. In the case of the Biomax membranes 4-5 times smaller than the NMWL is suggested. For more information on choosing the appropriate membrane cutt off: http://www.millipore.com/publications.nsf/docs/RF036 |
| What is the best way to store disc membranes? | Solutions of 10% ethanol in water or 0.1% azide in water will preserve the membrane. For long-term storage, refrigeration is recommended. |
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アミコン（Amicon）攪拌式セルＰｒｅｓｓｕｒｅ－ｂａｓｅｄ ｓａｍｐｌｅ ｃｏｎｃｅｎｔｒａｔｉｏｎ詳細はこちら >> |
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関連製品: Application Facete
| Protein Purification |
| Dialysis and Filtration |
関連製品: Brand Facete
| Ultracel® |
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| Reagents, Chemicals and Labware > Lab Filtration > Ultrafiltration Membrane Discs |