C6079
コラーゲナーゼ + プロテアーゼインヒビター
2-5 FALGPA units/mg solid, ≥800 CDU/mg solid
別名:
Collagenase preparation, Protease inhibitor solution
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biological source
bacterial (Clostridium histolyticum)
Quality Segment
form
solid
specific activity
≥800 CDU/mg solid, 2-5 FALGPA units/mg solid
mol wt
68-130 kDa
solubility
water: soluble
shipped in
dry ice
storage temp.
−20°C
Gene Information
rat ... LOC296948(296948)
Application
Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.
Biochem/physiol Actions
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.
Analysis Note
クロストリパイン活性および非特異的中性プロテア-ゼ活性も有しています。
Other Notes
膵臓組織におけるトリプシン酵素活性を制限するため、プロテア-ゼインヒビタ-を混合した、厳選したロットのコラゲナ-ゼType XIです。 この組成物は、いくつかの社外研究機関との共同研究により開発されました。
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Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
C6079-BULK: + C6079-1G: + C6079-500MG-PW: + C6079-5G: + C6079-500MG: + C6079-5G-PW: + C6079-VAR:
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