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ステリトップ（Steritop）フィルターユニット

ステリトップ（Steritop）ボトルトップフィルターユニットは、大きなろ過面積により、33 mmまたは45 mm口の吸引ボトルに水溶液を迅速にろ過することができます。ファネル内側の小さなタブで、プレフィルターを固定できます。汚染防止のために、ファネルを取り付けるときフィルター表面に触れないように、ボトル取り付けネジ部は少し奥まった構造になっています。

ステリトップ（Steritop）ボトルトップフィルターユニットは、大きなろ過面積により、33 mmまたは45 mm口の吸引ボトルに水溶液を迅速にろ過することができます。ファネル内側の小さなタブで、プレフィルターを固定できます。汚染防止のために、ファネルを取り付けるときフィルター表面に触れないように、ボトル取り付けネジ部は少し奥まった構造になっています。

概要

仕様

ご注文情報

高流量およびタンパク低吸着0.22 µmミリポア エクスプレス (PES) メンブレン装着ユニット、水溶液のろ過滅菌用並べ替えとフィルターをクリアする Show Filter
カタログ番号説明ファネル容量アウトレット接続パッケージ
SCGPS01RE吸引式ボトルトップフィルター 150 mL 33 mmネジ口 12 価格＆在庫状況を表示
SCGPS02REステリトップ（Steritop）-GP, 0.22 µm, ポリエーテルスルホン, 250 mL 33 mm, 放射線滅菌済 250 mL 33 mmネジ口 12 価格＆在庫状況を表示
SCGPS05REステリトップ（Steritop）-GP, 0.22 µm, ポリエーテルスルホン, 500 mL 33 mm, 放射線滅菌済 500 mL 33 mmネジ口 12 価格＆在庫状況を表示

* These products have been tested and validated for use in stem cell research applications. To determine their effects on Mouse Stem Cell growth or differentiation, three lots of Steritop-GP devices were used to filter media with LIF. Once filtered, this media was used to passage mouse stem cells five times to verify that Steritop-GP filtration does not impact pluripotency of mouse stem cells.

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Documentation

FAQ（よくある質問と回答）

質問回答
Are Stericup and Steritop units autoclavable?No. The highest temperature that the product can be used at is 50C ( 122F ).
Can I bubble point my Stericup/top?No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.
Do you have a filter for 100% serum?Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
For tissue culture media filtration which membrane should I use?The best membrane for this application is the Millipore Express membrane. It was designed specifically to filter tissue culture media with serum. It is low protein binding, has fast flow and high throughput (which is also called filter capacity).
I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol?The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.

Both Express and Durapore membranes are compatible. MCE is not.
I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this?All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.
Is the Express membrane surfactant free?Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Why use a vacuum driven filter rather than a pressure driven filter?Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
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カテゴリー

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices