04-928
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
culture supernatant, clone A3S, Upstate®
別名:
H3, Histone H3
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
A3S, monoclonal
Species reactivity:
human, rat, Saccharomyces cerevisiae, mouse, chicken
Application:
ChIP, WB
Citations:
22
biological source
rabbit
antibody form
culture supernatant
antibody product type
primary antibodies
clone
A3S, monoclonal
species reactivity
human, rat, Saccharomyces cerevisiae, mouse, chicken
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H3F3B(3021)
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General description
17 kDa
An antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.
Immunogen
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3
Application
Anti-Histone H3 Antibody, CT, pan, clone A3S is a Rabbit Monoclonal Antibody for detection of Histone H3 also known as Histone H3 & has been validated in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Biochem/physiol Actions
Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3.
Physical form
Cultured supernantant in 0.05% sodium azide
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Routinely evaluated by immunoblot analysis.
Other Notes
Replaces: 05-928
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
04-928:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Marcel Donnet et al.
Journal of colloid and interface science, 340(2), 218-224 (2009-10-10)
Thermodynamic solubility calculations are normally only related to thermodynamic equilibria in solution. In this paper, we extend the use of such solubility calculations to help elucidate possible precipitation reaction pathways during the entire reaction. We also estimate the interfacial energy
Huiyuan Zhang et al.
Nature communications, 5, 5798-5798 (2014-12-17)
The transcriptional regulator STAT3 curbs pro-inflammatory cytokine production mediated by NF-κB signalling in innate immune cells, yet the mechanism by which this occurs has been unclear. Here we identify STAT3 as a pivotal negative regulator of Ubc13, an E2 ubiquitin-conjugating
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
Briggs, S D, et al.
Genes & Development, 15, 3286-3295 (2001)
Jessica Kain et al.
Molecular metabolism, 53, 101291-101291 (2021-07-12)
Type II nuclear hormone receptors, including farnesoid X receptors (FXR), liver X receptors (LXR), and peroxisome proliferator-activated receptors (PPAR), which serve as drug targets for metabolic diseases, are permanently positioned in the nucleus and thought to be bound to DNA
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Xiao, Tiaojiang, et al.
Genes & Development, 17, 654-663 (2003)
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 04-928 | 04053252731419 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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