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Merck

SCC008

ReNcell VM Human Neural Progenitor Cell LIne

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

別名:

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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この商品について

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203

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製品名

ReNcell VM Human Neural Progenitor Cell LIne, ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

biological source

human

manufacturer/tradename

Chemicon®
ReNcell

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

liquid nitrogen

Quality Level

100

関連するカテゴリー

Analysis Note

Each lot of ReNcell VM cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities. Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Neural Stem Cells

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

General description

Product Source: Human ventral mesencephalon brain tissue
ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell VM was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. In experiments performed by the ReNeuron Group plc, ReNcell VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from ReNcell VM have furthermore been shown to be electophysiologically active. ReNcell VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell VM cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.

ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.
(Published in Nature)

Other Notes

> 1x106 viable ReNcell VM cells derived from human ventral mesencephalon brain tissue, cryopreserved. Store in liquid nitrogen.
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Preparation Note

When stored at the recommended storage conditions (liquid nitrogen), ReNcell VM cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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Chandrasekhar Kothapalli et al.
Biomaterials science, 8(19), 5452-5464 (2020-10-01)
While the mechanotransduction-induced fate of adult neural stem/progenitor cells (NPCs) is relatively known, how substrate stiffness regulates the temporal evolution of the biomechanics and phenotype of developmentally relevant human fetal NPCs (hNPCs) and their mechanosensing pathways remain unknown. Here, we
Jeremy D Hill et al.
Brain, behavior, and immunity, 76, 165-181 (2018-11-23)
New neurons are continuously produced by neural stem cells (NSCs) within the adult hippocampus. Numerous diseases, including major depressive disorder and HIV-1 associated neurocognitive disorder, are associated with decreased rates of adult neurogenesis. A hallmark of these conditions is a
Biljana Stangeland et al.
Oncotarget, 6(28), 26192-26215 (2015-08-22)
Glioblastoma (GBM) is both the most common and the most lethal primary brain tumor. It is thought that GBM stem cells (GSCs) are critically important in resistance to therapy. Therefore, there is a strong rationale to target these cells in
A three-dimensional human neural cell culture model of Alzheimer's disease.
Choi, SH; Kim, YH; Hebisch, M; Sliwinski, C; Lee, S; D'Avanzo, C; Chen, H; Hooli et al.
Nature null
C O Gigek et al.
Translational psychiatry, 5, e565-e565 (2015-05-13)
Genes implicated in neurodevelopmental disorders (NDDs) important in cognition and behavior may have convergent function and several cellular pathways have been implicated, including protein translational control, chromatin modification, and synapse assembly and maintenance. Here, we test the convergent effects of
関連する文献をすべて表示

資料

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

プロトコル

Human Neural Stem Cells Differentiation with TrueGel3D®

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

関連コンテンツ

Cellutions Newsletter: 2008, Volume 3
ReNcell®Human Neural Progenitors: Renewable and Consistent Supply of Human Functional Neurons

ReNcell® VM and ReNcell® CX are two well-established neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. This article describes the characteristics and differentiation of ReNcell® lines. Results of calcium and membrane potential changes in response to various ligands are also shown. The convenience to maintain in culture and flexibility to differentiate to individual scientists’ needs make ReNcell® lines the ideal platform for research and discovery.

Cellutions Newsletter: 2008, Volume 1

グローバルトレードアイテム番号

カタログ番号GTIN
SCC00804053252662713

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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