製品名
乳酸アッセイキット, sufficient for 100 colorimetric or fluorometric tests
usage
sufficient for 100 colorimetric or fluorometric tests
species reactivity
mouse, human
storage condition
dry at room temperature
technique(s)
activity assay: suitable
input
blood
cell
accession no.
P00338
NC_000011.10
NC_000073.7
application(s)
cosmetics
food and beverages
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
Application
乳酸アッセイキットは、サンプル中の乳酸の濃度を求めるために使用されています。
培養培地、発酵培地、血液および細胞中のL(+)-乳酸の検出に適しています。
Biochem/physiol Actions
このアッセイでは、乳酸濃度を酵素的に求めるため、存在する乳酸と比例して比色(570 nm)/蛍光(λex=535 nm/λem= 587 nm)の結果が得られます。このキットの感度の典型的なレベルは 0.001~10 mMです。
General description
L(+)-乳酸は、酵素である乳酸デヒドロゲナーゼの作用により動物の体内で生成される代謝化合物です。乳酸は、増殖中の細胞中および運動などの嫌気的条件下で産生されます。 異常に高い乳酸濃度は、癌、糖尿病、乳酸アシドーシスなどの病的状態と関連付けられています。L(+)-乳酸は、ヒトの中間代謝で生成される乳酸の主な立体異性体であり、約1~2 mmol/Lの濃度で血中に存在します。このキットは、生物学的サンプル中のL(+)-乳酸を検出する便利な手段になります。サンプルの前処理や精製の必要はありません。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
保管分類
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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資料
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
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