GenElute^™ 血液用ゲノムDNA抽出キット

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

GenElute^™ Blood Genomic DNA Kit has been used:

• to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits
• to isolate DNA from the blood samples 
• to extract whole blood DNA from the left blood cells

The purified genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

• 全血200μLからの標準収量は4-10μg。
• 24時間以上保存した血液からも採取直後の血液と同様にゲノミックDNAを精製可能。
• EDTA、ヘパリン、クエン酸ナトリウムなど多くの抗凝固薬と共に使用可能。
• 40分以内に全血から高純度のゲノミックDNA精製。
• 保存血液の夾雑物を完全に除去するprewash solutionを添付。
• A₂₆₀/A₂₈₀比が1.6~1.9の高純度ゲノミックDNAを抽出。
• RNase溶液がキットに添付。
• 最大50 kbまでのDNAを精製。
• 得られた精製DNAは、PCR、制限消化、サザンブロットにそのまま使用可能です。

詳細はwww.sigma-aldrich.com/genomicdnaをご覧ください。

GenElute is a trademark of Sigma-Aldrich Co. LLC