P9172
Pyronin Y
for NA electrophoresis
Synonym(s):
Pyronin Y, Pyronin G, Pyronin J
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About This Item
Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
Molecular Weight:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3795789
Colour Index Number:
45005
grade
Molecular Biology
Quality Segment
form
powder
composition
Dye content, ≥45%
technique(s)
nucleic acid detection: suitable
mp
250-260 °C (lit.)
solubility
water: 10 mg/mL, dark red
suitability
suitable for nucleic acid staining
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
InChI
1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
InChI key
INCIMLINXXICKS-UHFFFAOYSA-M
Application
Pyronin Y has been used:
- in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
- as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
- staining of CD34+ cells
Biochem/physiol Actions
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Preparation Note
This product is soluble in water 1 mg/ml, yielding a clear dark red solution.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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