Skip to Content
Merck

Key Documents

View All Documentation

S8820

Protease Inhibitor Cocktail

SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use

Synonym(s):

Protease Inhibitor Tablets, protease inhibitor

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
tablet
Solubility:
water: 1 tablet/100mL, clear to hazy, colorless
Storage temp.:
2-8°C
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

Quality Segment

200

biological source

bovine lung, bovine milk

form

tablet

solubility

water: 1 tablet/100mL, clear to hazy, colorless

storage temp.

2-8°C

General description

SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.

Application

SIGMAFAST Protease Inhibitor Tablets has been used:
  • to wash raw semen for membrane protein analysis
  • in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
  • as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
  • as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1β1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Biochem/physiol Actions

Inhibits serine, cysteine, aspartic, and metalloproteases.

Features and Benefits

  • Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
  • Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
  • Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
  • Tested and optimized for general use: proven performance and reliability for a wide range of applications.

Physical form

Tablet that is water soluble.

Preparation Note

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Other Notes

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Still not finding the right product?

Explore all of our products under SIGMAFAST™ Protease Inhibitor Tablets

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library