製品名
セルリティック™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
form
solution
storage temp.
room temp
Quality Level
関連するカテゴリー
Analysis Note
CelLytic Mは106~107個の浮遊細胞に125 μL使用します。接着細胞では500~1,000μL/100mmプレ-トあるいは200~400μL/35mmプレ-トで使用します。
Features and Benefits
- 効率:凍結融解や超音波破砕、他社製品よりも最高で50%効率的です。
- 非変性条件:免疫沈降、キナ-ゼアッセイ、ホスフォタ-ゼアッセイ、レポ-タ-遺伝子アッセイ、ゲルシフトアッセイ等その後のアプリケ-ションに影響を与えません。
- 便利:調製済みの試薬なので培養皿からの剥離が不要です。
- 迅速:室温で迅速に細胞溶解ができます。
General description
CelLytic Mは哺乳動物の培養細胞から全タンパク質を効率よく抽出するために調製された独自の界面活性剤です。浮遊細胞、接着細胞のどちらからでも迅速に細胞を溶解しタンパク質を抽出します。ライセ-トはレポ-タ-遺伝子アッセイ、ウェスタンブロット、免疫沈降、ゲルシフトアッセイ、ホスフォタ-ゼアッセイ、キナ-ゼアッセイ等のアプリケ-ションに適用可能で、その際のCelLytic Mの除去は不要です。
Preparation Note
細胞溶解M試薬のタンパク質抽出効率が、HeLa、CHO、COS、HL-60、Jurkat、A431、PC-12、ウシ大動脈内皮細胞(BAEC)などの細胞で試験されています。
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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資料
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
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