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R8755

RPMI-1640 Medium

With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820
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Product Name

RPMI-1640 Medium, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium is used for the following applications:
  • Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
  • Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • Used for cell isolation and culture
  • Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
  • Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • Used for the preparation of antifungal agents

Preparation Note

Formulated to contain 10.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R8755-50K: + R8755-50L: + R8755-25KG: + R8755-10L: + R8755-BULK: + R8755-VAR: + R8755LF1-BIBC: + R8755-1L: + R8755-2X5L: + R8755-BIBC: + R8755-10X1L: + R8755-PROC: + R8755-50KG: + R8755-25K:

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