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Merck

R7020

Transcript RNA Markers 0.2-10 kb

for RNA electrophoresis

Synonym(s):

RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105337
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−70°C
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grade

Molecular Biology

Quality Segment

form

liquid

usage

25 uses

solubility

water: soluble

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

General description

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.
Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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