Asia Pacific

Home

Campaign

e-workflow

Western Blot

western-blot-01.png

<a href="https://www.sigmaaldrich.com/KR/ko/campaigns/protein-research?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_lysisextraction_kr">Lysis &amp; Extraction Kits</a>
- Plant &amp; Yeast Cell:
&nbsp; &nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/bioactive-small-molecules?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_lysisextraction_kr">CelLytic™</a>&nbsp;/&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/protein-lysis-and-extraction/yeast-cell-lysis?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_lysisextraction_kr">PopCulture®</a>
- Bacterial Cell:
&nbsp; &nbsp;CelLytic™&nbsp;/&nbsp;<a href="https://www.sigmaaldrich.com/life-science/protein-sample-preparation/protein-extraction/bacterial-cell-lysis.html?utm_source=interactive-pdf&amp;utm_medium=Tools-for-Protein-Workflow&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraaction_lysis_kr">BlugBuster®</a>
- Mammalian Cell:
&nbsp; &nbsp;CelLytic™&nbsp;/&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/protein-lysis-and-extraction/cell-lysis?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_lysisextraction_kr">Cytobuster® &amp; PhosphoSafe®</a>
<a href="https://www.sigmaaldrich.com/KR/ko/products?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_protease_kr">Protease Inhibitor Cocktail®</a>
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=14865289&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_phosphatase_kr">Phosphatase Inhibitor Cocktail®</a>&nbsp;(준비중)
<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/amicon-ultra-centrifugal-filters?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_concentration_kr">Concentraion &amp; Buffer Exchange: Amicon®</a>
<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/western-blotting/mpagegels?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_sampleextraction_reagents_kr">Reagents for Western Blotting</a>

western-blot-02.png

<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/materials-science-and-engineering/drug-delivery/tools-for-pegylation?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_quantitation_bca_kr">BCA &amp; Bradford &amp; Lowry</a>
High Sensitive Products:
<a href="https://www.sigmaaldrich.com/KR/ko/applications/protein-biology/protein-quantitation?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_quantitation_quantipro_kr">QuanPro®</a>&nbsp;/&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/applications/protein-biology/protein-quantitation?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_quantitation_fluoroprofile_kr">FluoroProfile®</a>
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=9625289&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_quantitation_proteinstandard_kr">Protein Standard</a>&nbsp;(준비중)

western-blot-03.png

Precast Gels &amp; Buffers
-&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/molecular-biology-and-functional-genomics?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_trupage_kr">TruPAGE™</a>
-&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/western-blotting/mpagegels?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_mpage_kr">mPAGE™</a>
<a href="https://www.sigmaaldrich.com/KR/ko/products/molecular-biology-and-functional-genomics?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_acrylamides_kr">Acrylamides</a>&nbsp;&amp;&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/western-blotting/mpagegels?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_crosslinkers_kr">Cross-linkers</a>
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=9622722&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophore_gelcasting_kr">Gel Casting Reagents</a>&nbsp;(준비중)
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=19370931&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophore_reducing_kr">Reducing Agents</a>
<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/protein-electrophoresis-and-western-blotting/protein-markers#Peptide%20Marker?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_mwmakers_kr">MW Markers</a>
<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/protein-electrophoresis-and-western-blotting/protein-electrophoresis-gel-stains?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_coomassie_kr">Coomassie Stains</a>
<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/protein-electrophoresis-and-western-blotting/protein-electrophoresis-gels-and-buffers?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_electrophoresis_buffernreagents_kr">Electrophresis &amp; Transfer Buffers &amp; Reagents</a>

<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/protein-electrophoresis-and-western-blotting/immunodetection-reagents-and-substrates?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_detection_chemiluminescent_kr">Chemiluminescent Substrates&nbsp;&amp;&nbsp;ReBlot™</a>
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=14561556&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_detection_chromogenic_kr">Chromogenic Substrates</a>&nbsp;(준비중)
<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/genomics/pcr/troubleshooting?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_detection_films_kr">Detection Films</a>
<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/western-blotting/western-blotting?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_detection_wbprotocol_kr">★WB Protocol</a>
<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/genomics/pcr/troubleshooting?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_detection_wbtroubleshooting_kr">★WB Troubleshooting</a>

<a href="https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/protein-biology/western-blotting/western-blotting-with-snap-id-system?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_snap_kr">SNAP i.d. ® 2.0 Protein Detection System</a>
<a href="https://www.sigmaaldrich.com/KR/ko/products/cell-culture-and-analysis/cell-culture-media-and-buffers?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_buffers_kr">Blocking Buffers</a>&nbsp;&amp;&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-reagents?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_reagents_kr">Reagents</a>&nbsp;&amp;&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_bsa_kr">BSA</a>
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=14561863&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_washbuffers_kr">Wash Buffers</a>
<a href="https://www.sigmaaldrich.com/life-science/cell-biology/antibodies.html?&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">Antibody</a>
-&nbsp;<a href="https://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-products.html?TablePage=13837785&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">Primary (Monoclonal&nbsp;&amp;&nbsp;Polyclonal)</a>
-&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/antibodies/secondary-antibodies?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">Secondary</a>&nbsp;(<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/antibodies/secondary-antibodies?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">Speices</a>&nbsp;&amp;&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/antibodies?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">Conjugate</a>)
-&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/antibodies/zoomab-antibodies?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">ZooMAb (Recombinant Antibody)</a>
-&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/products/protein-biology/antibodies?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_antibody_kr">Antibody Lebeling Kit</a>
-&nbsp;<a href="https://www.sigmaaldrich.com/KR/ko/program/biogurantee-program?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_blocking_guarantee_kr">개런티 프로그램</a>

<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=14561752&amp;utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_proteintransfer_immobilon_kr">Immobilon® PVDF Transfer Membrane</a>
<a href="https://www.sigmaaldrich.com/KR/ko/labware/labware-products.html?TablePage=17198752?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_proteintransfer_immobilon_kr">Immobilon® NC Trasnfer Membrane</a>
<a href="https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=14561862?utm_source=e-workflow&amp;utm_medium=microsite&amp;utm_campaign=westernblot_proteintransfer_stains_kr">Protein Blot Stains</a>

<h1>Western Blotting
</h1>

aem-GridColumn aem-GridColumn--default--12

Western Blotting

Sample Extraction & Preparation

Quantitation

Electrophoresis

Protein Transfer

Blocking & Antibody Incubation

Detection