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Merck

G6392

L-Glutamine

γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):

(S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide

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About This Item

Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
56-85-9
Molecular Weight:
146.14
MDL number:
MFCD00008044
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24895254
EC Number:
200-292-1
NACRES:
NA.75
Beilstein/REAXYS Number:
1723797

Key Documents

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Product Name

L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

sterility

γ-irradiated

product line

BioXtra

form

powder

packaging

vial of 0.292 g

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to faint yellow

mp

185 °C (dec.) (lit.)

solubility

H2O: 10 mL/vial, clear to slightly hazy

Quality Level

200

Gene Information

rat ... Ggt1(116568)

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Application

L-Glutamine has been used as a media supplement:
  • for cochlear organotypic culturing
  • in Iscove′s modified Dulbecco′s medium for HeLa cells culturing
  • in Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing

Qualified for use in cell culture applications, especially serum-free cell culture.

Biochem/physiol Actions

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.
L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.

Other Notes

The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.

Packaging

50 mL amber serum vial with rubber stopper.

Preparation Note

Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF8950
SLCB7305
SLBW7296
SLBR7287V
SLBP8447V

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The signal peptide anchors apolipoprotein M in plasma lipoproteins and prevents rapid clearance of apolipoprotein M from plasma
Christoffersen C, et al.
The Journal of Biological Chemistry, 283(27), 18765-18772 (2008)
Cisplatin ototoxicity in rat cochlear organotypic cultures
Ding D, et al.
Hearing Research, 282(1-2), 196-203 (2011)
Nonresonant confocal Raman imaging of DNA and protein distribution in apoptotic cells
Uzunbajakava N, et al.
Biophysical Journal, 84(6), 3968-3981 (2003)
Rebekah M Samsonraj et al.
Stem cells (Dayton, Ohio), 33(6), 1878-1891 (2015-03-11)
This study sought to identify critical determinants of mesenchymal stem cell (MSC) potency using in vitro and in vivo attributes of cells isolated from the bone marrow of age- and sex-matched donors. Adherence to plastic was not indicative of potency
Pablo Hernández-Varas et al.
Nature communications, 6, 7524-7524 (2015-06-26)
Cell-matrix adhesions are central mediators of mechanotransduction, yet the interplay between force and adhesion regulation remains unclear. Here we use live cell imaging to map time-dependent cross-correlations between vinculin-mediated tension and adhesion complex area, revealing a plastic, context-dependent relationship. Interestingly
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Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

Protocols

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

WST-1 Assay Protocol for Cell Viability

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

세포 생존성 및 증식을 위한 MTT 분석 프로토콜

세포 생존성, 증식, 세포독성 측정을 위한 MTT 분석 프로토콜. MTT 시약 조제에 대한 지침 및 응용분야 예시.

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