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Merck

L0543

LB Agar Kanamycin-50, Plates

pre-poured LB agar plates with kanamycin sulfate

Synonym(s):

kanamycin agar plates

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About This Item

UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli, selective for coliforms

Key Documents

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Molecular Biology

sterility

sterile; autoclaved

form

plates (10 cm), plates (10cm, 20-25ml each)

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli, selective for coliforms

Quality Level

200

Related Categories

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Other Notes

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCQ5579
MKCQ3897
MKCQ4681
MKCQ2251
MKCQ1003

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Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
Deich RA, et al.
Journal of Bacteriology, 170(2), 489-498 (1988)
Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles
Talhinhas P, et al.
Molecular Biotechnology, 39(1), 57-67 (2008)

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