SEQR
SeqPlex RNA Amplification Kit
For use with high throughput sequencing technologies
Synonym(s):
WGA kit, Whole transcriptome amplification
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About This Item
NACRES:
NA.55
UNSPSC Code:
41121800
Quality Level
technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Application
SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.
Features and Benefits
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Protocols
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.